Figure 5
Abbrogation of anti-oxidant effect of apelin by overexpression of FoxO3-TM in H9C2 cells.
(A) H9C2 cells were transfected to express GFP (in green) and the dominant negative mutant FoxO3-TM. Cells were submitted to hypoxic conditions (H) or kept in normoxia (N) in the presence or not of apelin (A). Mitochondrial O2− production was measured by confocal microscopy using MitoSOX (in red) in transfected cells and in non-transfected cells (NT) as a control. Bar is 10μm. (B) Quantification of MitSOX fluorescence per cells from (A). §§§p < 0.001 vs N from transfected cells; ###p < 0.001 vs H from NT cells; ns, non significant.
