Figure 6
Apelin treatment reverses FoxO3 nuclear translocation induced by hypoxia in cardiac cells through a PI3-K dependent pathway.
(A) Representative confocal images of cardiomyocytes isolated from HFD-fed mice stimulated or not by apelin for 15 minutes (A, 10−7M), submitted to hypoxic (H) or normoxic (N) conditions for 2 hours and stained in green for FoxO3. Nuclei were stained with DAPI (in blue). Bar is 20 μm. (B) Quantification of FoxO3 nuclear translocation from (A,C) H9C2 cells treated with the PI3-K inhibitor LY294002 (LY) or with the p38/MAPK SB203580 (SB) in the presence (A) or absence of apelin and then submitted to hypoxic conditions (H) or left in normoxia (N) for 2 h. Cells were fixed and stained with an anti-FoxO3 antibody (in green), nuclei were stained with DAPI (in blue). Bar is 10 μm. (D) Quantification of FoxO3 nuclear translocation was done as in (B). *p < 0.05 vs N; ***p < 0.001 vs N; #p < 0.001 vs H; §p < 0.001 vs H+A.
