Figure 8
FoxO3 nuclear translocation in mice hearts subjected to I/R is abrogated by post-reperfusion injection of apelin.
(A) Heart sections from HFD-fed mice treated or not with apelin (A) and sham-operated (S) or submitted to I/R were immunostained for FoxO3 (in green). Nuclei were stained with DAPI (in blue). Bar is 20 μm. (B) Quantification of FoxO3 nuclear translocation from (A). *p<0.05 vs S ***p < 0.001 vs S; #p < 0.05 vs I/R. (C) Immunostaining of FoxO3 (in green) on heart sections from HFD-fed WT or apelin KO mice. Nuclei were stained with DAPI (in blue). Arrows point to nuclei devoid of FoxO3, whereas arrowheads point to nuclear staining.
