Figure 5
From: A novel 2,5-diaminopyrimidine-based affinity probe for Bruton’s tyrosine kinase
Probe 14 is a selective affinity probe for Btk.
(a) Concentration-dependent labeling of recombinant Btk by probe 14; (b) time-dependent labeling of recombinant Btk by probe 14; (c) probe 14 predominantly labeled endogenous Btk in live cells (concentration course); (d) time course experiments in cellular labeling; (e) immunoprecipitation of Btk from probe 14-labeled lysates. lane 1: cell lysates; lane 2: supernatant after removal of intrinsic IgG; lane 3: supernatant after immunoprecipitation; lane 4: supernatant of the last wash before elution; lane 5: supernatant of the first elution by applying LDS sample buffer onto protein A Sepharose beads.
