Table 1 Motility parameters of human spermatozoa under various conditions.

From: Involvement of opsins in mammalian sperm thermotaxis

Treatment (n)

VSL (μm/s)

VCL (μm/s)

VAP (μm/s)

LIN (%)

FD

MOT (%)

10 μM M119K (8)

76 ± 3*

95 ± 5*

78 ± 4*

81 ± 2*

1.27 ± 0.02

54 ± 7*

7 μM M119K (16)

86 ± 4

108 ± 5*

92 ± 4

80 ± 2*

1.19 ± 0.03

66 ± 4

3 μM M119K (9)

82 ± 2

116 ± 4

92 ± 3

71 ± 2

1.23 ± 0.02

68 ± 3

1 μM M119K (10)

85 ± 3

120 ± 4

96 ± 3

71 ± 2

1.24 ± 0.02

63 ± 3

Control (28)

85 ± 3

125 ± 3

99 ± 3

68 ± 2

1.31 ± 0.03

67 ± 3

NNC (21)

67 ± 4

101 ± 5*

75 ± 4*

66 ± 2

1.33 ± 0.03*

68 ± 4

Control (25)

76 ± 2

111 ± 3

87 ± 2

69 ± 2

1.27 ± 0.01

70 ± 3

SKF96365 (10)

85 ± 8

118 ± 8

96 ± 9

71 ± 5

1.24 ± 0.05

77 ± 5

Control (15)

84 ± 4

119 ± 5

96 ± 5

70 ± 2

1.26 ± 0.03

66 ± 5

Pyr3 (8)

95 ± 4

130 ± 4

107 ± 3

74 ± 3

1.27 ± 0.06

73 ± 3

Control (10)

89 ± 3

127 ± 5

101 ± 3

71 ± 4

1.31 ± 0.04

69 ± 5

NH2OH dark (12)

65 ± 3*

102 ± 5*

74 ± 5*

64 ± 2

1.20 ± 0.01*

66 ± 5

NH2OH light (10)

60 ± 4*

100 ± 6*

68 ± 5*

60 ± 2

1.19 ± 0.01*

73 ± 4

Light (10)

80 ± 3

123 ± 6

95 ± 5

65 ± 2

1.30 ± 0.03

71 ± 4

Control (18)

78 ± 2

123 ± 4

93 ± 3

64 ± 1

1.31 ± 0.03

67 ± 4

NH2OH dark (4)

58 ± 6*

91 ± 5*

64 ± 6*

63 ± 3

1.17 ± 0.01

70 ± 8

NH2OH 400 nm (5)

63 ± 5*

99 ± 4

71 ± 5

64 ± 4

1.22 ± 0.03

66 ± 10

400 nm (5)

78 ± 3

117 ± 2

89 ± 3

66 ± 2

1.25 ± 0.01

74 ± 6

Control (5)

74 ± 4

113 ± 5

87 ± 6

65 ± 1

1.29 ± 0.04

71 ± 6

NH2OH dark (9)

64 ± 4*

97 ± 4*

71 ± 4*

65 ± 2

1.19 ± 0.01*

74 ± 3

NH2OH 560 nm (9)

66 ± 3

102 ± 4*

74 ± 3*

65 ± 2

1.22 ± 0.02*

65 ± 5

560 nm (6)

81 ± 4

125 ± 6

96 ± 4

65 ± 2

1.28 ± 0.02

68 ± 7

Control (9)

74 ± 2

114 ± 5

86 ± 3

66 ± 2

1.32 ± 0.02

64 ± 4

NH2OH dark (5)

55 ± 5*

85 ± 6*

61 ± 6*

64 ± 4

1.18 ± 0.03

68 ± 6

NH2OH 630 nm (5)

64 ± 5

95 ± 5

70 ± 5

67 ± 4

1.18 ± 0.05

75 ± 4

630 nm (5)

83 ± 4

118 ± 5

94 ± 3

70 ± 3

1.27 ± 0.07

65 ± 5

Control (6)

75 ± 4

106 ± 4

84 ± 4

71 ± 4

1.29 ± 0.04

71 ± 5

NH2OH dark (5)

64 ± 4

94 ± 5

70 ± 4

67 ± 1

1.18 ± 0.04

63 ± 4

NH2OH 730 nm (5)

65 ± 8

99 ± 8

72 ± 9

65 ± 4

1.22 ± 0.02

68 ± 9

730 nm (5)

76 ± 6

115 ± 8

88 ± 7

66 ± 3

1.31 ± 0.03

63 ± 7

Control (5)

75 ± 4

110 ± 6

87 ± 5

68 ± 2

1.28 ± 0.05

63 ± 2

IBMX (9)

62 ± 6

115 ± 6*

77 ± 6

54 ± 4*

1.35 ± 0.05*

61 ± 6

Control (8)

75 ± 4

101 ± 3

82 ± 4

74 ± 3

1.19 ± 0.01

63 ± 6

Caffeine (8)

85 ± 7

134 ± 6

107 ± 7

63 ± 4*

1.28 ± 0.06

60 ± 5

Control (9)

84 ± 5

115 ± 8

97 ± 7

74 ± 4

1.24 ± 0.06

50 ± 4

Sildenafil (6)

98 ± 8

120 ± 6

108 ± 8

81 ± 3

1.13 ± 0.03

67 ± 6

Control (10)

98 ± 5

121 ± 5

108 ± 6

81 ± 2

1.11 ± 0.03

64 ± 5

IBMX (6)

50 ± 5

125 ± 12

59 ± 6

40 ± 3*

1.28 ± 0.04*

64 ± 7

2APB (6)

40 ± 4*

126 ± 17

49 ± 5*

32 ± 1*

1.30 ± 0.03*

63 ± 8

IBMX + 2APB (6)

32 ± 2*

139 ± 12*

44 ± 3*

24 ± 2*

1.6 ± 0.1*

58 ± 9

Control (6)

57 ± 8

110 ± 12

65 ± 9

51 ± 3

1.17 ± 0.01

61 ± 8

  1. Human sperm samples were subjected to different drugs and treatments, detailed in the text and then the motility was analysed (37 °C). Concentrations: 10 μM NNC, 1 μM SKF96365, 1 μM Pyr3, 5 mM hydroxylamine, 1mM IBMX, 2 mM caffeine, 0.1 μM sildenafil, 0.1 mM 2APB. Abbreviations: NH2OH, hydroxylamine; VSL, straight-line velocity (the time-average velocity of the sperm head along a straight line from its first position to its last position, expressed in μm/s); VCL, curvilinear velocity (time-averaged velocity of a sperm head along its actual curvilinear path, expressed in μm/s); VAP, average path velocity (velocity over an average path generated by a roaming average, expressed in μm/s), LIN, linearity [defined as (VSL/VCL)×100]60; FD, fractal dimension (a quantitative measure of the intensity of hyperactivation, expressing the degree to which the sperm trajectory fills a plane)37; MOT (%), percentage of motile spermatozoa. The values of VSL and MOT are in bold because the sperm accumulation in the warmer compartment in thermotaxis assays shown in Fig. 1a is expected to be linearly dependent on these parameters. The values are the mean ± SEM of the indicated n determinations (i.e., aliquots; 300–1800 cells for each aliquot). *P < 0.05 according to two-way ANOVA (relative to the non-treated control).
Back to article page