Table 2 Effect of hydroxylamine on temperature-dependent hyperactivation of human spermatozoa.

From: Involvement of opsins in mammalian sperm thermotaxis

Treatment

T(°C)

FD

%HYP

No hydroxylamine

34

1.25 ± 0.01

13 ± 2

37

1.17 ± 0.01*

4 ± 1*

With hydroxylamine

34

1.16 ± 0.01

2 ± 1

37

1.15 ± 0.02

1.9 ± 0.5

  1. The motility parameters FD and percentage of hyperactivated spermatozoa (%HYP) were consecutively analysed at 34 °C and 37 °C for 1 min periods under the illumination of a bright-field microscope in the presence and absence of hydroxylamine (5 mM). Each of the values shown is the mean ± SEM of 12 determinations. *P < 0.0001 according to two-way ANOVA for the difference from 34 °C.
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