Figure 7 | Scientific Reports

Figure 7

From: MiR-146a-5p suppresses activation and proliferation of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis through directly targeting Wnt1 and Wnt5a

Figure 7

Knockdown of Wnt1 or Wnt5a inhibits Wnt signaling pathway and reduces HSC activation.

(A) Knockdown efficiency of Wnt1 or Wnt5a in HSC-T6 cells was confirmed by RT-PCR and (B) Western blot. (C) Knockdown of Wnt1 or Wnt5a inhibits the mRNA and protein expressions (D) of β-catenin, NFAT5 and increase the expression of GSK-3β. (E) Knockdown of Wnt1 or Wnt5a inhibits the mRNA and protein levels (F) of α-SMA, Col-1 and MMP-2 and enhanced the expression of Smad7. β-actin was used as loading control. Values are mean ± SD, *P < 0.05, **P < 0.01 compared with control.

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