Table 1 Primers for real-time quantitative PCR analysis.
Gene | Length of production (bp) | Primers |
|---|---|---|
Wnt1 | 116 | F 5′- CTGTGCGAGAGTGCAAATGG -3′ |
R 5′- GATGAACGCTGTTTCTCGGC -3′ | ||
Wnt1-3′UTR | 919 | F 5′- CCGCTCGAGCCCTCACGACCTCGTCTACT -3′ |
R 5′- ATTTGCGGCCGCGGCCACATTTCGGTGCAAAT -3′ | ||
Wnt1-3′UTR mut | 919 | F 5′- GCTCTCTTCCGACGAGTCGACACACTCGCTGGTC -3′ |
R 5′- CGAGTGTGTCCTGAGCAGGAAGAGAGCGTTCCCG -3′ | ||
Wnt5a | 621 | F 5′- GTGATGCAAATAGGCAGCCG -3′ |
R 5′- GTCCATCCCCTCTGAGGTCT -3′ | ||
Wnt5a-3′UTR | 472 | F 5′- CCGCTCGAGACAGTTTTCTCTGGGCTTGC -3′ |
R 5′- ATTTGCGGCCGCCACTTTTGAAACAGAGGTGCCC -3′ | ||
Wnt5a-3′UTR mut | 472 | F 5′- GCCCATGGCCGAGCAGTCACCCTCTCTTTGGTGT-3′ |
R 5′- AGAGAGGGTGTGACGAGGGCCATGGGCAGGAAGC-3′ | ||
β-catenin | 137 | F 5′- CAGATCCCATCCACGCAGTT -3′ |
R 5′- ATTGCACGTGTGGCAAGTTC -3′ | ||
GSK-3β | 158 | F 5′- CGAACTCCACCAGAGGCAAT -3′ |
R 5′- AAGAGTGCAGGTGTGTCTCG -3′ | ||
NAFT5 | 178 | F 5′- TCTCACACATCCAGACCCCT -3′ |
R 5′- TGTACAAAGGCTCTGTCGCT -3′ | ||
α-SMA | 98 | F 5′- CTGACAGAGGCACCACTGAA-3′ |
R 5′- CATCTCCAGAGTCCAGCACA-3′ | ||
Col-1 | 308 | F 5′- GGGCGAGTGCTGTGCTTT -3′ |
R 5′- GACCCATTGGACCTGAACC -3′ | ||
MMP-2 | 103 | F 5′- TTTCTATGGCTGCCCCAAGG -3′ |
R 5′- GTCAAGGTCACCTGTCTGGG -3′ | ||
Smad7 | 153 | F 5′- TTTAAACCCGGGCTCCATCC -3′ |
R 5′- GGAGGGGACCGAGTAGACTT -3′ | ||
GAPDH | 233 | F 5′- GGTGAAGGTCGGTGTGAACG -3′ |
R 5′- CTCGCTCCTGGAAGATGGTG -3′ |
