Figure 4
Representative image of synaptophysin immunolabelling in the spinal cord ventral horn after 2 weeks of lesion.
((A) 10×) The Ipsilateral (IL) of the treated group has stronger synaptophysin labeling than ((B) 10×) the Ipsilateral of non-treated group. ((C,D) 10×) The Contralateral of both groups showed similar synaptophysin immunolabeling pattern. (E–H) A representative motor neuron image analyzed by Start LSM Image Examiner (Carl Zeiss AIM Software) at zoom (5×). On surface of motor neuron at (E) the Ipsilateral of the treated group, the synaptophysin immunolabeling are better p reserved when compared to (F) Ipsilateral non-treated-group moto neuron, but less preserved than the (G,H) Contralateral motor neuron of both groups. (I–L) The Integrated density of pixels obtained by Start LSM Image Examiner (Carl Zeiss AIM Software) emphasizes the difference among moto neurons. (M) Graph represents the quantification of the immunolabeling in all groups (**p < 0.01).
