Figure 2
Stepwise image processing of an OF test by MouseMove.
(a) Video frame of a mouse during OF testing. (b) Video frame of the empty OF. (c) Screenshot of MouseMove’s preprocessing macro into which the two videos are entered. (d) Same frame as in ‘Panel (a)’ but after binary thresholding. (e) Same frame as in ‘Panel (b)’ but after binary thresholding. The yellow rings in ‘Panels (d,e)’ indicate the perimeter of the OF as detected by MouseMove, which allows automatic spatial alignment of the two videos. (f) Same frame from ‘Panel (a)’ but after subtraction of ‘Panel (e)’ from ‘Panel (d)’. ‘1 = 153,72’ stipulates the assigned number of the measurable object (i.e. one) and its arbitrary x,y coordinates at that point in time (i.e. x = 153 and y = 72). (g) Cumulative trajectories of the same mouse over 100 s in the OF. (h) Screenshot of MouseMove’s graphical user interface (GUI) and visual depiction of the analyses performed by MouseMove for an OF test. (i) Example of MouseMove’s text file output for the OF testing of three representative MCAo-operated mice.
