Figure 5
Analysis of laterality differences in mice after experimental stroke using MouseMove.
(a) Schematics showing how MouseMove determines whether a mouse is turning left or right at each location (Pi). The algorithm first determines the vector at Pi (red) and at a prior time point (Pi-1; green). If the angle change from Pi-1 to Pi is Δθi>0, then the mouse is turning left. If the angle change from Pi−1 to Pi is Δθi <0, then the mouse is turning right. (b) The ratio of left-turns versus right-turns (LRatio; dots plotted against the left-hand y-axis) and the laterality offset (the absolute value of 1-LRatio; bar graph plotted against the right-hand y-axis) for MCAo-operated (n = 7) and sham-operated (n = 6) mice over a 400  s period. ****p < 0.0001 as determined by two-sided unpaired t-test for the differences in laterality offset. (c) Schematic showing how MouseMove determines the trajectory at a point (P) and how the curvature radius refers to the inner tangential circle radius (red). (d) Trajectory curvature radius for sham-operated (n = 6) and MCAo-operated (n = 7) mice over a 400 s period. Dot points represent the mean value for individual mice. Line and error bars represent the cohort mean ± s.e.m. *p < 0.05 as determined by two-sided unpaired t-test. These results were derived from the same videos as were used in Fig. 4.
