Figure 2 | Scientific Reports

Figure 2

From: Ventral tegmental area dopamine and GABA neurons: Physiological properties and expression of mRNA for endocannabinoid biosynthetic elements

Figure 2

Gene expression profiles of ventral tegmental area dopaminergic and GABAergic neurons.

(A) A representative rat DAergic cell expressing DAGLα, 12LO, DAT, mGluR5 and NAPE-PLD. (B) A representative rat GABAergic neuron expressing DAGLα, mGluR1, 12LO, NAPE-PLD, mGluR5 and GAD67. (C) A representative mouse DAergic neuron expressing TH, DAGLα and mGluR5. (D) A representative mouse GABAergic neuron expressing GAD67, NAPE-PLD, mGluR1, mGluR5, DAGLα and 12LO. Relative fluorescence was generated by FAM-TAMRA probes and graphed relative to the number of PCR cycles performed. Inset: Gel electrophoresis from the PCR reaction of a mouse GAD67-GFP neuron demonstrating the expression of 12LO (100 bp), DAGLα (100 bp), NAPE-PLD (104 bp), mGluR1 (78 bp) and mGluR5 (103 bp). Inset: Gel electrophoresis from a PCR reaction demonstrating cell-type specific expression of TH/DAT and GAD65/67 within rat DA (D) and GABA (G) neurons. Amplicon sizes for PCR products are TH: 107 bp; DAT: 101 bp; GAD65: 155 bp; GAD67: 73 bp.

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