Figure 5 | Scientific Reports

Figure 5

From: Ventral tegmental area dopamine and GABA neurons: Physiological properties and expression of mRNA for endocannabinoid biosynthetic elements

Figure 5

Triple immunolabeling of DAGLα and mGluR5 within VTA DAergic and GABAergic neurons.

Immunofluorescence showing DAGLα and mGluR5 distribution in VTA neurons, with clear labeling in the cytosol of most DA and some GABA neurons. (A) TH immunolabeling, (B) GAD67-GFP labeling, (C) DAGLα immunolabeling, (D) Merged images. (E) TH immunolabeling, (F) GAD67-GFP labeling, (G) mGluR5 immunolabeling, (H) Merged images. All images were taken at 60× magnification and were acquired from slices taken from 2 animals. Closed arrowheads: TH-positive target-positive labeled neuron; open arrowheads: GAD67-GFP target-positive labeled GABA neuron; arrow: GFP-positive, target-negative neuron. Asterisk: Non-specific blood vessel labeling that occurred in mGluR5 immunostaining experiments. Scale bars: 100 μm.

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