Figure 2

ZFP36L1 is validated as a potential regulator in monocytic differentiation.

(a) Expression profile of ZFP36L1 in AML patients and monocytic differentiation derived from the array data. (b) Real-time PCR analysis of ZFP36L1 mRNA expression in peripheral blood MNCs derived from 56 AML patients (FAB M1 to M5 subtypes) and 57 normal controls. (c) Western blot analysis of ZFP36L1 protein level in 12 AML patients and 12 normal controls. (d) Real-time PCR and western blot analyses of ZFP36L1 expression during PMA-induced monocyte/macrophage differentiation of THP-1 cells. (e) Real-time PCR and western blot analyses of ZFP36L1 expression during PMA-induced monocyte/macrophage differentiation of HL-60 cells. (f) Real-time PCR and western blot analyses of ZFP36L1 expression during the in vitro monocytic induction culture of CD34⁺ HSPCs.