Figure 4

Validation of ZFP36L1’s role in monocyte/macrophage differentiation of CD34⁺ HSPCs.

(a,b) The expression of ZFP36L1 was analyzed using real-time PCR (a) and western blot (b) during the in vitro monocytic induction culture of CD34⁺ HSPCs infected with lenti-shZFP36L1 or lenti-ctrl. (c) Expression of the monocytic differentiation marker CD14 was evaluated by flow cytometry after the infection and induction of CD34⁺ HSPCs. A representative experiment is presented in the left and the results from two independent experiments were statistically analyzed and presented as mean ± SD in the right. The red line and the black line indicate untreated cells and CD14 antibody-stained cells respectively. (d) Real-time PCR analysis of CD14 and CSF1R mRNA expression during the differentiation of CD34⁺ HSPCs infected with lenti-shZFP36L1 or lenti-ctrl. (e) May-Grünwald Giemsa staining of the infected and differentiation-induced CD34⁺ HSPCs. The cells were observed under ×40 magnification. A representative field for each experiment is presented in the left and a statistical analysis of the differentiated monocyte/macrophages by counting cells in five fields is presented in the right. Data are represented as mean ± SD. *P < 0.05 and **P < 0.01, Student’s t-test.