Figure 7

ZFP36L1 regulates monocyte/macrophage differentiation by targeting CDK6.

Rescue assays were performed by infection with a combination of lenti-ZFP36L1 (or lenti-ctrl) and lenti-CDK6 (or lenti-ctrl) in CD34⁺ HSPCs followed by monocytic induction for 20 days. The cells in 16 days of differentiation were collected for further analyses. (a) The levels of ZFP36L1 and CDK6 were determined by western blot. (b) Real-time PCR of CD14 and CD11B mRNA expression. (c) CD14 expression was evaluated through flow cytometry analysis and a statistical analysis of two independent experiments is shown in the bottom panel. The red line and the black line indicate untreated cells and CD14 antibody stained cells respectively. *P < 0.05 and **P < 0.01, Student’s t-test.