Figure 6
From: Nanoliposomal Nitroglycerin Exerts Potent Anti-Inflammatory Effects
NTG-NL Enhances Endothelial NO Production.
(A) Immunofluorescent staining of ECs labeled with NO-sensitive dye (DAF-FM diacetate) and (B) subsequent image analysis (bar graph; n = at least 30 cells) confirms that, like free NTG, NTG-NL enhances NO production in L-NIO-treated cells. Scale bar: 25 μm. **p < 0.01; ***p < 0.001; ns, no significance. (C) Immunofluorescent staining of U937 cell-EC co-cultures for ICAM-1 and subsequent fluorescent intensity measurement (bar graph; n = at least 20 cells) reveals that, like free NTG (5 μM), NTG-NL (5 μg/ml) suppresses ICAM-1 clustering induced by NO deficiency (L-NIO treatment). **p < 0.01; ***p < 0.001; ns, no significance. Data are expressed as mean ± SEM.
