Figure 2

The HFD mice were more vulnerable to oxidative damage and the antioxidant capacity was decreased.

(A) The DCF average fluorescent intensity was presented in histogram, the ROS production was significantly increased in HFD mice. (B) The MDA production was significantly increased in HFD mice. Antioxidant capacity, including T-AOC activities (C) and SOD activities (D) were measured. Both of them were significantly decreased in HFD mice. ATP production (E) was significantly increased in the HFD mice. Mitochondrial content (F) were similar between HFD mice and SD mice. The level of mitochondria-related miR-141-3p (G) was strikingly increased and the levels of miR-196a-5p, miR-210-3p and miR-378a-3p were significantly decreased in the liver of 16-week-old HFD mice compared with 16-week-old SD mice. The level of mitochondria-related miR-141-3p (H) was markedly increased in the 16-weel-old HFD mice compared with the 8-week-old mice. Each data point represented the mean ± SE from three separate experiments in which treatments were performed in triplicate. *P < 0.05.