Figure 1

Expression of Slc15a1 in adult rat testes.
(A) Gene expression of Slc15a1 in adult rat testes (T), Sertoli cells (SC), germ cells (GC), peritubular myoid cells (MC) and interstitial Leydig cells (LC) was analyzed by RT-PCR. S-16 served as a control. M, DNA ladder marker. (B) Immunoblotting of Slc15a1 in lysates of T, SC, GC, MC and LC (∼50 μg proteins/lane) with actin as a loading control. Histogram summarizes the immunoblot results after normalizing each data point against corresponding actin. Slc15a1 protein level in the testis was arbitrarily set as 1. Bars represent means ± sd, n = 3. *P < 0.05, **P < 0.01. n.s., not detectable.