Table 1 Primers used for RT-PCR in this study.

From: Slc15a1 is involved in the transport of synthetic F5-peptide into the seminiferous epithelium in adult rat testes

Gene

Primer sequence

Orientation

Position

Length (bp)

A.T. (°C)

Cycle no.

GenBank®accession no.

Slc15a1

5′- CTCTGCTACCTGACTCCAA-3′

sense

184–202

223

53

25

NM_057121

 

5′- TACCAAGGGCTATCAGGG-3′

anti-sense

389–406

    

S-16

5′- TCCGCTGCAGTCCGTTCAAGTCTT-3′

sense

15–38

385

53

25

XM_341815

 

5′- GCCAAACTTCTTGGTTTCGCAGCG-3′

anti-sense

376–399

    
  1. A.T., annealing temperature.