Table 1 Primers used for RT-PCR in this study.
Gene | Primer sequence | Orientation | Position | Length (bp) | A.T. (°C) | Cycle no. | GenBank®accession no. |
---|---|---|---|---|---|---|---|
Slc15a1 | 5′- CTCTGCTACCTGACTCCAA-3′ | sense | 184–202 | 223 | 53 | 25 | NM_057121 |
5′- TACCAAGGGCTATCAGGG-3′ | anti-sense | 389–406 | |||||
S-16 | 5′- TCCGCTGCAGTCCGTTCAAGTCTT-3′ | sense | 15–38 | 385 | 53 | 25 | XM_341815 |
5′- GCCAAACTTCTTGGTTTCGCAGCG-3′ | anti-sense | 376–399 |