Figure 5

Expression profile and effect of somatostatin analogues on GH secretion and cell proliferation in cultures from GC tumors.

(A) Expression profile of somatostatin receptor (Sstr) subtypes in GC tumors (n = 5). mRNA copy number was adjusted by expression of a control gene (beta-actin) (B) Effect of 4 h treatment with somatostatin analogues (SOM-230, Octreotide and BIM-23120) at 100 nM on GH secretion in cultures from GC tumors. Values are expressed as percent of fetal bovine serum (FBS)-treated control cells (set at 100%; n = 3; 3–5 wells/treatment). (C) Cell viability in culture of GC tumors treated with somatostatin analogues (SOM-230, Octreotide and BIM-23120) at 100 nM for 24, 48 and 72 hours as compared with FBS-treated control cells. Values are expressed as percent of FBS-treated controls (set at 100%; n = 3; 3–5 wells/treatment). Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001.