Figure 3
From: Immuno-analysis of microparticles: probing at the limits of detection
A direct correlation between MP size and marker expression.
(a) Immuno-gold labelled MP grids were imaged at 60 000X Mag with the JEOL 1400 TEM. All images are to the same scale where scale bars are measured in micron. Closed arrow-heads indicate gold positive MP whilst open arrow-heads indicate gold-negative MP. (b) Spearman’s correlation shows a significant relationship between the size of MP, measured as the maximum Feret diameter (μm) and the number of gold particles bound per MP for the annexin V label (r = 0.68; p < 0.0001). (c) The median size, measured as the maximum Feret diameter in μm, was compared between gold-positive and gold-negative MP for each of the markers of interest (Mann Whitney test; ***p < 0.0001).
