Table 1 The numbers and percentages of MP as assessed by FCM.
From: Immuno-analysis of microparticles: probing at the limits of detection
NS | TNF | NS | TNF | |
|---|---|---|---|---|
Annexin V | Lactadherin | |||
CD54 and PS dual labelled samples (mean MP number/103 cells ± SEM) | ||||
CD54 | 3.7 ± 0.3 | 9.1 ± 0.8 | 3.2 ± 0.3 | 9.7 ± 1.5 |
PS | 129.8 ± 5.8 | 235.1 ± 2.6* | 108 ± 5 | 186 ± 6.9* |
Double | 78.9 ± 3.4 | 220.9 ± 14.4* | 88.2 ± 3.3 | 239.2 ± 14.9* |
Total | 212.5 ± 8.6 | 465.1 ± 21.2* | 199.4 ± 7.4 | 435.4 ± 16.2* |
CD105 and PS dual labelled samples (mean MP number/103 cells ± SEM) | ||||
CD105 | 8.2 ± 0.5 | 8.3 ± 0.3 | 9.87 ± 1.8 | 22.5 ± 3.7* |
PS | 158 ± 10.8 | 347.3 ± 9.8* | 121.2 ± 5.8 | 250.3 ± 12.4* |
Double | 61.5 ± 2.5 | 130.1 ± 7.4* | 78.1 ± 3.6 | 185.1 ± 9.7* |
Total | 231 ± 11.3 | 485.8 ± 3.7* | 209.2 ± 8.9 | 457.9 ± 23.2* |
Fluorescence positivity for individual labels (% positive (% range)) | ||||
PS | 31 (24–44) | 48 (41–62) | 44 (8–69) | 52 (21–72) |
CD54# | NS: 17 (5–34) | TNF: 25 (17–45) | ||
CD105# | NS: 13 (3–32) | TNF: 17 (9–33) | ||
Fluorescence positivity for multiple labels (% positive ± SEM) | ||||
CD54/PS | 37 ± 0.8 | 47 ± 1.6 | 44 ± 0.9 | 54 ± 1.7 |
CD105/PS | 28.4 ± 1.5 | 27.1 ± 1.1 | 37.6 ± 1.2 | 37.1 ± 1.3 |
