Figure 1 | Scientific Reports

Figure 1

From: Rapid identification of haloarchaea and methanoarchaea using the matrix assisted laser desorption/ionization time-of-flight mass spectrometry

Figure 1

Comparison of the haloarchaea, Haloarcula hispanica ATCC 33960T mass spectra from samples that used different extraction methods.

(a) The cell pellet was suspended in sterile water and then ethanol was added to precipitate the peptides/proteins. The pellet was suspended in 70% FA then ACN was added to give a final concentration of 50%. (b) The cell pellet was suspended in sterile water then EA was added and mixed thoroughly. The EA layer was discarded and ethanol was added to the water layer to precipitate the peptides/proteins. The pellet was suspended in 70% FA then ACN was added to give a final concentration of 50%. (c) A similar procedure as outlined in b was used, but ethanol was substituted by acetone to precipitate the peptides/proteins. (d) The cell pellet was directly suspended in 75% ethanol followed by the same procedure as outlined in a (e) The cell pellet was directly suspended 75% ethanol then the precipitated pellet was extracted by ACN.

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