Table 1 List of haloarchaea and methanoarchaea used in this study as well as the growth conditions, accession numbers of 16S rRNA genes and re-identified scores.

From: Rapid identification of haloarchaea and methanoarchaea using the matrix assisted laser desorption/ionization time-of-flight mass spectrometry

Organism	Medium	NaCl (%)	Methanogenesis substrate	Accession number	Re-identified score
Haloarchaea
Haloarcula argentinensis ATCC 700875^T	NHA	24	–	NR_116080	2.673 ± 0.019
Haloarcula hispanica ATCC 33960^T	NHA	24	–	NR_113419	2.601 ± 0.033
Haloarcula japonica ATCC 49778^T	NHA	24	–	NR_116082	2.314 ± 0.067
Haloarcula marismortui ATCC 43049^T	NHA	24	–	NR_121590, AY596298^a, NR_074201	2.504 ± 0.025
Haloarcula quadrata ATCC 700850^T	NHA	24	–	EF645689, EF645694	2.479 ± 0.016
Haloarcula vallismortis ATCC 29715^T	NHA	24	–	NR_113424	2.569 ± 0.018
Natrinema pellirubrum JCM 10476^T	NHA	24	–	NR_113528	2.141 ± 0.054
Haloterrigena turkmenica DSM 5511^T	NHA	24	–	NR_074238	2.272 ± 0.016
Haloterrigena thermotolerans DSM 11552^T	NHA	24	–	NR_113514	2.715 ± 0.030
Haloterrigena saccharevitans JCM 12889^T	NHA	24	–	NR_113512	2.709 ± 0.014
Haloterrigena hispanica DSM 18328^T	NHA	24	–	NR_113508	2.692 ± 0.036
Haloarcula sp. HLR1	NHA	24	–	DQ089681	2.414 ± 0.004
Haloarcula sp. HLR2	NHA	24	–	DQ089682	2.403 ± 0.031
Haloarcula sp. HLR3	NHA	24	–	KP326318	2.196 ± 0.037
Haloarcula sp. HLR4	NHA	24	–	DQ089683	2.378 ± 0.017
Haloarcula sp. HLR5	NHA	24	–	DQ089684, DQ089685	2.263 ± 0.052
Haloarcula sp. HLR6	NHA	24	–	DQ089686	2.218 ± 0.128
Natrinema sp. HLW1	NHA	24	–	KP326314	2.528 ± 0.049
Natrinema sp. HLW2	NHA	24	–	KP326315	2.564 ± 0.042
Natrinema sp. HLW3	NHA	24	–	KP326316	2.405 ± 0.020
Natrinema sp. HLW4	NHA	24	–	KP326317	2.550 ± 0.029
Haloterrigena sp. H1	NHA	24	–	AF478471	2.402 ± 0.077
Haloterrigena sp. H4	NHA	24	–	AY546002	2.380 ± 0.103
Haloterrigena sp. H5	NHA	24	–	KP702942	2.755 ± 0.025
Haloterrigena sp. H6	NHA	24	–	AY546107	2.725 ± 0.048
Haloterrigena sp. H7	NHA	24	–	KP702943	2.535 ± 0.060
Haloterrigena sp. H8	NHA	24	–	KP702944	2.582 ± 0.055
Haloterrigena sp. H9	NHA	24	–	KP702945	2.662 ± 0.060
Haloterrigena sp. H10	NHA	24	–	KP702946	2.470 ± 0.085
Haloterrigena sp. H11	NHA	24	–	KP702947	2.413 ± 0.037
Haloterrigena sp. H12	NHA	24	–	KP702948	2.558 ± 0.015
Haloterrigena sp. H13	NHA	24	–	AY055733	2.390 ± 0.009
Halophilic methanoarchaea
Methanohalophilus portucalensis FDF1^T	H-P	12	40 mM trimethylamine	NR_042826	2.676 ± 0.015
Methanohalophilus portucalensis FDF2	H-P	12	40 mM trimethylamine	KT285318	2.655 ± 0.010
Methanohalophilus sp. RET-1	H-P	12	40 mM trimethylamine	KT285314	2.552 ± 0.028
Methanohalophilus sp. SF-1	H-P	12	40 mM trimethylamine	KT285312	2.669 ± 0.035
Methanohalophilus sp. SF-2	H-P	12	40 mM trimethylamine	KT285313	2.597 ± 0.027
Methanohalophilus portucalensis Z-7302	H-P	12	40 mM trimethylamine	KT285311	2.370 ± 0.019
Methanohalophilus portucalensis Z-7401	H-P	12	40 mM trimethylamine	KT285317	2.561 ± 0.037
Methanohalophilus sp. Z-7404	H-P	12	40 mM trimethylamine	KT285315	2.573 ± 0.016
Methanohalophilus portucalensis Z-7301	H-P	12	40 mM trimethylamine	KT285316	2.506 ± 0.005
Methanohalophilus mahii DSM 5219^T	H-P	12	40 mM trimethylamine	M59133	2.350 ± 0.047
Methanococcoides methylutens Cas-1	H-P	4	40 mM trimethylamine	KT285309	2.667 ± 0.019
Methanolobus taylorii GS-16 ^T	H-P	4	40 mM trimethylamine	KT285308	2.640 ± 0.026
Methanohalobium evestigatum SD-1	H-P	12	40 mM trimethylamine	KT285310	2.297 ± 0.018
Halotolerant and non-halophilic methanoarchaea
Methanolobus vulcani DSM 3029^T	MB/W	0.5	50 mM methanol	NR_044768	2.574 ± 0.047
Methanolobus chelungpuianus St545Mb^T	MB/W	0.5	50 mM methanol	EU293796	2.312 ± 0.003
Methanofollis formosanus ML15^T	MB/W	0.5	50 mM formate + 20 mM acetate	NR_042767	2.594 ± 0.047
Methanofollis aquaemaris N2F9704^T	MB/W	0.5	50 mM formate + 20 mM acetate	NR_041801	2.671 ± 0.011
Methanocorpusculum sinense DSM 4274^T	MB/W	0.5	50 mM formate + 20 mM acetate	NR_117148	2.193 ± 0.089
Methanocorpusculum parvum DSM 3823^T	MB/W	0.5	50 mM formate + 20 mM acetate	NR_044728	2.501 ± 0.030
Methanocorpusculum parvum IF9601b	MB/W	0.5	50 mM formate + 20 mM acetate	AY057068	2.444 ± 0.023
Methanocorpusculum labreanum DSM 4855^T	MB/W	0.5	50 mM formate + 20 mM acetate	AY260436	2.441 ± 0.019
Methanocorpusculum bavaricum DSM 4179^T	MB/W	0.5	50 mM formate + 20 mM acetate	AY196676	2.475 ± 0.044
Methanoculleus chikugoensis Afa-1	MB/W	0.5	50 mM formate + 20 mM acetate	KP702949	2.731 ± 0.013
Methanobacterium palustre FG694aF	MB/W	0.5	50 mM formate + 20 mM acetate	EU293795	2.302 ± 0.020
Methanosarcina mazei O1M9701	MB/W	0.5	50 mM methanol	AF411469	2.649 ± 0.008
Methanosarcina mazei O1M9704b	MB/W	0.5	50 mM methanol	AF411467	2.471 ± 0.021
Methanosarcina mazei O1M9702	MB/W	0.5	50 mM methanol	AF411468	2.783 ± 0.006
Methanosarcina mazei FA9604c	MB/W	0.5	40 mM TMA + 20 mM acetate	AF262036	2.615 ± 0.063
Methanococcus voltaei P2F9701a	MB/W	0.5	50 mM formate + 20 mM acetate	AF306670	2.266 ± 0.041
Methanocalculus taiwanensis P2F9705	MB/W	0.5	50 mM formate + 20 mM acetate	AF411470	2.609 ± 0.036
Methanocalculus taiwanensis P2F9704a^T	MB/W	0.5	50 mM formate + 20 mM acetate	AF172443	2.600 ± 0.038
Methanocalculus pumilus MHT-1^T	MB/W	0.5	50 mM formate + 20 mM acetate	NR_028148	2.751 ± 0.035
Methanocalculus halotolerans SEBR 4845^T	MB/W	5.0	50 mM formate + 20 mM acetate	NR_024870	2.713 ± 0.024
Methanocalculus sp. O1F9702c	MB/W	0.5	50 mM formate + 20 mM acetate	AY026256	2.446 ± 0.038
Methanocalculus chunghsingensis K1F9705b^T	MB/W	0.5	50 mM formate + 20 mM acetate	NR_041828	2.490 ± 0.043
Methanocalculus chunghsingensis O1F9704a	MB/W	0.5	50 mM formate + 20 mM acetate	AY234332	2.540 ± 0.065
Methanocalculus chunghsingensis K1F9705c	MB/W	0.5	50 mM formate + 20 mM acetate	AF321115	2.520 ± 0.020

^aThe sequence of rrnaB was revealed from whole genome sequence.

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Table 1 List of haloarchaea and methanoarchaea used in this study as well as the growth conditions, accession numbers of 16S rRNA genes and re-identified scores.

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