Table 2 Tentative assignment of putative biomarkers from MALDI-TOF MS signals of haloarchaea type strains and methanoarchaea with genome data.

From: Rapid identification of haloarchaea and methanoarchaea using the matrix assisted laser desorption/ionization time-of-flight mass spectrometry

Species

UniProt Accession ID

Predicted Mass (Da)

Observed Mass (Da)/Intensity (%)

Error (%)

Protein Description

Peptide sequences

Haloarcula argentinensis ATCC 700875T

M0KI28

6143.86

6148.52/100

0.07

Uncharacterized protein

MVHCPDCETSLETADDIDFVEVDAVTGFIKASKRFYTANCAACGVTIGSGVA GAKSNGGAA

Haloarcula hispanica ATCC 33960T

G0HR47

6151.92

6148.08/100

0.06

Uncharacterized protein

MVHCPDCQTSLETADDIEFVEVDAKTGLIKASKRFYTANCAACGVTIGSGVA GAKSNGGAA

Haloarcula japonica ATCC 49778T

EMA34946a

6144.84

6148.70/84

0.06

hypothetical protein C444_00145

MVHCPDCQTSLETADDIDFVEVDATTGFIKASKRFYTANCAACGVTIGSGVA GAKSNGGAA

Haloarcula marismortui ATCC 43049T

Q5V7R0

6152.00

6147.87/100

0.07

Uncharacterized protein

MPEFRVRKPDGWTTVSFPDEVATISVVGGKVDGQLCLTFTGEREGGTSVVLD RLLPS

 

YP_137385a

6159.86

6164.05/71

0.07

hypothetical protein rrnAC2944

MVHCPDCETSLETADDIEFVEVDATTGFIKASKRFYTANCAACGVTIGSGVAG AKSNGGAA

Haloarcula vallismortis ATCC 29715T

M0JP93

6148.31

6148.62/100

0.01

Uncharacterized protein

MVARLYSATLFALYQLTLLLGIMLLPVAMVTEQFGLRLPMDRAVSGLNEAYD QASA

 

M0JI28

6187.91

6165.92/47

0.36

Uncharacterized protein

MVHCPDCETSLETADDIEFVEVDAVTGFIKASKRFYTANCATCGVTIGSGVAG AKSNGGAA

Natrinema pellirubrum JCM 10476T

L0JI54

6025.64

6030.57/100

0,08

Uncharacterized protein

MTPEATPVGREADRASDVVGAVDEIDGRPHLVVADIARDDAWIAMAESAAV AVEDHR

Haloterrigena turkmenica DSM 5511T

D2RXY3

6034.80

6045.45/100

0.17

Uncharacterized protein

MSTTSPVFCYVCNEEMVLDETLEHHLVYEHKPRELAKQLVAEWEAEELGEAV

 

YP_ 003403049a

6127.76

6070.09/24

0.93

hypothetical protein Htur_1490

MTLEAESVGSVSVTDGDVVAAIDEIGGQPHLVIADIGRDDVWLSMTERDAVS LDEWR

Haloterrigena thermotolerans DSM 11552T

M0BND2

6039.66

6031.49/100

0.14

Uncharacterized protein

MTPEATPVGREADRASDVVAAVDEIDGRPHLVVADIARDDAWIAMAESAAV AVEDHR

Methanosarcina mazei FA9604c

Q8PYQ4b

10679.59

10684.8/34

0.04

50S ribosomal protein L31e

MVGKMADDMVKEQIYTIPLREVRKVPAWKRAGRAVKEVRGFLVRH MKTEAEQVKLDKTINECLWEKGCEKPPLSIRVRAVKFADGEVQAELAQ

Methanococcus voltaei P2F9701a

D7DR73c

9764.07

9751.67/43

0.13

50S ribosomal protein L12

MEYIYAALLLNSADKEITEDAVKAVLTAAGIEADDARVKALVAALEGVDIAE AIAKAAAAPVAVAAAAPAAEAPAEEKKEEKKEDTGAAAAAGLGALFG

 

D7DV66c

9740.40

9751.67/43

0.12

50S ribosomal protein L31e

MENERIYTIPLRDVTNKVPTTKRAPRAIKKIREYLQKHMKSDNVKLDNSIN EKVWERSLNKIPARVRVKAVKQDDVVIATLVE

Methanohalophilus mahii DSM 5219T

D5E990

11436.11

11433.53/20

0.02

UPF0235 protein Mmah_0207

MPIRDAIHTKGNGCIIDFEINPGSSKLVVPSGYNIWRKRVEGKLTESAQKGK ANDQLIQRLSHIFQINSSSITIVAGAKTTKKSVHLENVYPKTAEDVLEQYL

  1. aProtein accession number from NCBI protein database.
  2. bThe protein was described from Methanosarcina mazei Gö1T.
  3. cThe protein was described from Methanococcus voltaei A3T.
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