Figure 3

Nucleosomal apyrimidinic DNA binding of UV-DDB.

(a) Schematic representations of reconstituted nucleosomes containing undamaged DNA, AP(inside) and AP(outside). (b) Gel electrophoretic mobility shift assay for nucleosome binding of UV-DDB. Nucleosome core particles (NCP; 5 nM) containing undamaged DNA (lanes 1–6), AP(inside) (lanes 7–12), or AP(outside) (lanes 13–18) were incubated with UV-DDB. The UV-DDB concentrations are 0 nM (lanes 1, 7 and 13), 2.5 nM (lanes 2, 8, and 14), 5 nM (lanes 3, 9 and 15), 10 nM (lanes 4, 10, and 16), 20 nM (lanes 5, 11 and 17) and 40 nM (lanes 6, 12 and 18). (c) Graphic representation of the experiments shown in panel (b). Standard deviation values are shown (n = 3). (d) Graphic representation of naked DNA binding of UV-DDB. Standard deviation values are shown (n = 3).