Figure 5
From: SENP1 desensitizes hypoxic ovarian cancer cells to cisplatin by up-regulating HIF-1α
SENP1 decreases the sensitivity of SKOV3 cells to cisplatin in hypoxia.
SKOV3 cells transfected with pl-SENP1 or si-SENP1 were treated with 60 μM cisplatin for 48 hours. (A) Flow cytometry showed that pl-SENP1 significantly decreased the cell apoptosis rate while si-SENP1 significantly upregulated the cell apoptosis rate in hypoxia (P < 0.05). However, this phenomenon was not obvious in normoxia. Trypan blue exclusion assay (B) and CCK8 assay (C), N = normoxia H = hypoxia) showed the similar results as in (A,D) Western blot showed that SENP1 upregulated the protein level of MDR1 and downregulated the protein level of PARP especially in hypoxia.
