Figure 5

Cardiac performance after transplantation of LPA-treated CD34⁺ cells.

Myocardial infarctions were induced by permanent ligation of the left anterior descending coronary artery (LAD). (A) Representative echocardiographic images of left ventricles before (Normal), after myocardial infarction without and with Gel + cells + LPA treatment. (B.1,C.1) Cardiac fractional shortening (B.1) or ejection fraction (C.1) in animals with (PBS, Gel + cells, Gel + cells + LPA) or without ligation of left anterior descending coronary artery (Normal). In LPA-treated CD34⁺ cells, the cells were treated with 100 μM of LPA, 1 h before transplantation. Cells (1 × 10⁶ cells) were delivered in a fibrin gel precursor solution (200 μL) into the infarcted heart of nude rats. (B.2,C.2) Cardiac fractional shortening (B.2) and ejection fraction (C.2) at week 3 was normalized by week 1. In graphs (B,C) results are presented as mean ± SEM (n = 6–10). *Denotes statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.