Figure 5
From: Conversion of a light-driven proton pump into a light-gated ion channel
Inward-directed photocurrents produced by CsR-R83Q are channel-like.
(a) Position W182 is highly conserved in microbial rhodopsins and is located close to the methyl groups of the retinal. (b) Photocurrents of CsR-W182F are characterized by a large transient outward peak compared to the stationary currents. (c) IStat(E) of stationary photocurrents of CsR-W182F are always directed outward but curved compared to CsR-WT (550 ± 25 nm, normalized to pH 7.5 and 0 mV, pH 10 [n = 4], pH 7.5 [n = 6], pH 5 [n = 5]). (d) Similar to CsR-R83Q, photocurrents of CsR-R83Q-W182F are inward-directed, especially at low pHo. Additionally, the currents show drastically increased decay times. Data were measured in oocytes and represent the mean ± SE.
