Figure 5

PolyIC and LPS increase IL-1α/IL-1β induced secretion of inflammatory mediators in infra-patellar fat pad derived fibroblasts.

Fibroblasts isolated from infra-patellar fat pads (n = 5) were stimulated with IL-1α, IL-1β (both 500 pg/ml), LPS, PolyIC (both 5 μg/ml) or HMGB-1 (50 ng/ml) alone or in combination for 24 hours and the secretion of IL-8, IL-6 and GM-CSF quantified by ELISA. (a,b) IL-1α and IL-1β induced secretion of GMCSF was accentuated by PolyIC. In contrast, Poly IC induced only an additive effect on IL-1α and IL-1β induced secretion of IL-6 and IL-8. LPS induced an additive effect on IL-1α and IL-1β induced secretion of GMCSF, IL-6 and IL-8 while HMGB1 had no effect. (c) Co-treatment with HMGB-1 and LPS or PolyIC was not significantly different to treatment with LPS or PolyIC alone. Data presented as mean ± standard error of the mean.