Figure 8

Increased expression of α-SMA positive cells in tissue from revision TKA.

(a) Representative images of α-SMA stained synovial membrane (upper panel) and infrapatellar fat pad (lower panel) from patients undergoing primary TKA (left panel), patients undergoing non-arthrofibrotic revision TKA (middle panel) and patients undergoing arthrofibrotic revision TKA (right panel). α-SMA staining is increase in revision TKA tissue compared to primary TKA tissue. Images acquired on a Nikon inverted microscope at 20x magnification. (b) α-SMA positive staining was quantified using image analysis software. There was a significant increase in α-SMA positive staining of synovial membrane (i) and infrapatellar fat pad (ii) from revision patients compared to primary TKA regardless of clinical diagnosis. There was no significant difference in α-SMA positive staining of either tissue between patients undergoing non-arthrofibrotic revision TKA and patients undergoing arthrofibrotic revision TKA. Synovial membrane and Infrapatellar fat pad; primary TKA n = 10, non-arthrofibrotic revision TKA n = 5, arthrofibrotic revision TKA n = 5. *p < 0.05. ***p < 0.001.