Figure 2

Brain section loaded with 5 mM FeCl3 and stained with Prussian blue reaction (PBR).
(A) Light microscopy image of a neuron with a PN on which iron is accumulated. (B) Corresponding quantitative PIXE element image of phosphorus (green) and iron (red). The PN shows prominent iron accumulation (red). Cellular somata are identified by the phosphorus signal (green). Scale bar: 20 μm.