Figure 2
Expression analyses of intestinal transporters in the human iPS-derived enterocyte-like cells.
Human iPS-derived enterocyte-like cells (hiPS-ELCs) were differentiated according to the protocol described in Fig. 1E. (A) The gene expression levels of PEPT1 in Caco-2 cells, hiPS-ELCs and Adult Intestine were measured by real-time RT-PCR analysis. On the y axis, the gene expression levels in Caco-2 cells were taken as 1.0. (B) The hiPS-ELCs were assayed for expression of PEPT1 (Red) by immunohistochemistry. Nuclei were stained with DAPI (Blue). Scale bars represent 40 μm. (C,D) The gene expression levels of apical transporters (C) and basolateral transporters (D) in the Caco-2 cells, hiPS-ELCs and Adult Intestine were measured by real-time RT-PCR analysis. On the y axis, the gene expression levels in Adult Intestine were taken as 1.0. Data are represented as the means ± S.E. (n ≧ 3). Statistical analysis was performed using the unpaired two-tailed student’s t-test. *P < 0.05. (E) After the enterocyte differentiation, the hiPS-ELCs were treated with or without 100 μM captopril for 24 hr. The hiPS-ELCs were treated with D-Ala-Leu-Lys-AMCA (blue) for 4 hr. After the uptake of D-Ala-Leu-Lys-AMCA, the cells were fixed and stained with anti-VILLIN antibodies (green). Scale bars represent 50 μm.
