Figure 3
CYP3A4 expression level and induction potency in the human iPS-derived enterocyte-like cells.
Human iPS cell-derived enterocyte-like cells (hiPS-ELCs) were differentiated according to the protocol described in Fig. 1E. (A) The gene expression levels of CYP3A4 in Caco-2 cells, hiPS-ELCs and Adult Intestine were measured by real-time RT-PCR analysis. On the y axis, the gene expression level in Caco-2 cells was taken as 1.0. (B) The CYP3A4 protein expression levels in the Caco-2 cells and hiPS-ELCs were measured by western blotting analysis. (C) The CYP3A4 activity levels in the Caco-2 cells and hiPS-ELCs were measured by CYP3A4-Glo assay kit. (D,E) The CYP3A4 induction potency was examined in the hiPS-ELCs and Caco-2 cells. The hiPS-ELCs and Caco-2 cells were treated with 100 nM 1,25-dihidroxyvitamin D3 (VD3) (D) or 20 μM rifampicin (RIF) (E) for 24 hr or 48 hr, respectively and then the gene expression levels of CYP3A4 were measured by real-time RT-PCR analysis. On the y axis, the gene expression levels of CYP3A4 in Caco-2 cells treated with DMSO (Solvent) were taken as 1.0. (F,G) The gene expression levels of VDR (F) and PXR (G) in the hiPS-ELCs and Caco-2 cells were examined by real-time RT-PCR analysis. On the y axis, the gene expression levels of VDR and PXR in Caco-2 cells were taken as 1.0. The data are represented as the means ± S.E. (n ≧ 3).
