Figure 4
Inhibition of Crosstalk between HSC and HCC Cells via the Suppression of TIMP-1 Secretion by TGF-β Blockade.
(A) Effects of EW-7197 on the CM-induced proliferation of SK-HEP1, SNU354 and HepG2 cells. Cells were treated with the indicated concentration of EW-7197 in CM for 72 h. (B) A schematic for the identification of the effects of EW-7197 on CM-treated cells. (C) Densitometric analysis (up) and representative images (down) of wound-healing assay of SK-HEP1 cells. Cells were treated with CM in the presence or absence of EW-7197 (1 μM) for 30 h. Scale bars: 200 μm. (D) Representative images (left) and densitometric analysis (right) of soft agar assay of SK-HEP1 cells. Cells were treated with CM in the presence or absence of EW-7197 (1 μM) for 24 h. After treatment, the cells were counted and placed in soft agar for colony assay to determine cell survival. ***p < 0.001 vs. untreated control, #p < 0.05 vs. CM-treated control and ###p < 0.001 vs. CM-treated control.
