Figure 1
Abnormal cell morphology and proliferation of the Spag6-deficient (KO) MEFs.
Representative images of MEFs from a wild-type mouse (a) and a Spag6-deficient mouse (b) under a light microscopy. Notice that Spag6-deficient MEFs were larger than the wild-type MEFs. There seems to be more vesicles in the mutant cells (arrows in b). Relative cell surface area was measured on MEFs from three wild-type and three Spag6-deficient mice, 50 cells were analyzed from each line. The cell surface of Spag6-deficient MEFs was significantly greater than that of the wild-type MEFs (c). *p < 0.05. Immortalized (d) and passage two (e) wild-type and Spag6-deficient MEFs were seeded in 24 well plates and cell numbers were counted for five continuous days. The data presented are the average from three wild-type and three mutant lines. Notice that the Spag6-deficient MEFs grew at a much slower rate than the wild-type MEFs in both immortalized and passage two cells.
