Figure 5
Multiple centrosomes in the Spag6-deficient (KO) MEFs.
(a) Representative images of immortalized wild-type MEFs double stained with a monoclonal anti-γ tubulin antibody. Only two centrosomes were present in each wild-type MEFs. (b) Representative images of immortalized Spag6-deficient MEFs showing multiple centrosomes and these centrosomes are not localized at the correct position. Frequently, abnormal large nuclei was seen in the Spag6-deficient MEFs (The arrow-heads point to abnormally large nuclei). (c) Representative images of passage two wild-type (left) and Spag6-deficient (right) MEFs stained with anti-γ tubulin antibody. Only two centrosomes were present in each wild-type MEF, but multiple centrosomes were observed in some Spag6-deficient MEFs. White arrows point to the centrosomes. (d) Quantitation of multiple centrosomes (>2) in primary and immortalized wild-type and Spag6-deficient MEFs. 500 cells were counted for each cell line and three wild-type and three KO MEFs were analyzed. The percentages of cells with multiple centrosomes are significantly higher in the Spag6-deficient MEFs. *p < 0.05.
