Figure 8
Forced-expression of SPAG6 rescues abnormal morphology observed in the Spag6-deficient (KO) MEFs.
(a) Examination of cell morphology by light microscopy. Notice that before infection, the two Spag6-deficient MEFs had a large surface area. One day after infection, the cells treated with control virus maintained the same morphology (right panel). However, the cells infected with SPAG6-expressing virus showed reduced size and changed to a fibroblast-like morphology. (b) Western blot analysis showed that once infected with the SPAG6-expression virus, the Spag6-deficient MEFs expressed both SPAG6 protein and GFP, an indicator of virus infection. Expression of acetylated tubulin was also increased. The cells infected with control virus only expressed GFP and the cells without any virus treatment expressed neither GFP nor SPAG6. (c) Immunofluorescence labeling images of the Spag6-deficient cells infected with control virus and SPAG6-expressing virus. Notice that the cells infected with control virus expressed GFP only and the GFP positive area is larger than the cells expressing SPAG6, indicating a larger cell size. The cells expressing SPAG6 (arrows) have smaller nuclei than the non-SPAG6 expressing cells (arrow heads).
