Figure 2 | Scientific Reports

Figure 2

From: Rapid whole genome sequencing of Miyazaki-Bali/2007 Pteropine orthoreovirus by modified rolling circular amplification with adaptor ligation – next generation sequencing

Figure 2

Schematic diagram for the genomic sequence assembly of PRV Miyazaki-Bali/2007 strain based on sequencing data obtained by mRCA-NGS.

Raw sequence data was first assembled and aligned to the genomic segment sequence of other orthoreovirus strains (used as reference sequences) obtained through GenBank® using GS Reference Mapper software with minimal overlap identity modified from 90% to 40%. The resulting draft sequences were subsequently cleaned-up; a process now simplified by the easy identification of the MI-A3′ linker sequence. Upon sequence verification, the trimming of the MI-A3′ adaptor sequence was performed as a final step in the sequence assembly to generate the final sequences.

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