Table 1 List of nine selected viruses present in the mock-virome together with their classification, host species and physical characteristics.

From: Modular approach to customise sample preparation procedures for viral metagenomics: a reproducible protocol for virome analysis

Virus/phage strain

Virus family

Host

Baltimore classification

Shape

Genome composition

Genome size (kb)

Virion size (nm)

Enveloped?

Bouyant Density in CsCl (g cm−2)

Number of viral genome copies/mL

Porcine circovirus 2 (10-10)

Circoviridae

Pigs

Group II: ssDNA

Spherical

Circular

1.8

≈17

NO

1.37

1.62 × 109

Feline panleukopenia virus

Parvoviridae

Cats

Group II: ssDNA

Spherical

Linear

5

≈20

NO

1.40

2.09 × 1010

Polyomavirus (BKV)

Polyomaviridae

Humans

Group I: dsDNA

Spherical

Circular

5

≈50

NO

1.34

1.34 × 109

Pepino Mosaic virus (CH2)

Alphaflexiviridae

Tomato plant

Group IV: ssRNA (+)

Rod

Linear

6

≈470-800  ×  12–13

NO

1.31

1.34 × 109

Rotavirus A (WC3)

Reoviridae

Cows

Group III: dsRNA

Spherical

Segmented

19

≈100

NO

1.36–1.39

8.57 × 108

Feline infectious peritonitis virus (serotype 1683)

Coronaviridae

Cats

Group IV: ssRNA (+)

Spherical

Linear

30

≈100

YES

1.23–1.24

8.76 × 108

Bovine herpesvirus 1 (Cooper strain)

Herpesvirus

Cattle

Group I: dsDNA

Spherical

Linear

135

≈200

YES

1.23

4.16 × 107

LIMEstone (vB_DsoM_LIMEstone1)

Myoviridae

Bacterium Dickeya solani (potato pathogen)

Group I: dsDNA

Head-tail structure

Linear

152

≈91 (head), 114 × 17 (tail)

NO

1.50

1.69 × 108

Acanthamoeba polyphaga mimivirus

Mimiviridae

Acanthamoeba polyphaga (Amoeba)

Group I: dsDNA

Spherical

Linear

1181

≈500-700

YES (Internal)

1.36

1.47 × 108