Figure 1

Rab32 Facilitates the Efficiency of Reprogramming.

(a) Schematic representation of iPSC generation with different factor combinations. (b,d) The reprogramming efficiencies of factor combinations on the induction of iPSCs were quantified by determining the number of Oct4-GFP⁺ colonies in mES medium (b) and 10% KOSR medium (d) n = 4, the results of OSK+Ctr were used as control. *p < 0.05; **p < 0.01 by general linear model. (c,e) Full-well mosaic images of Oct4-GFP⁺ are shown for OSKCtr, OSKR and OSKN at day 16 and AP⁺ colonies at day 18 in mES medium (c) and 10% KOSR medium (e). (f) Characterization of Oct4-GFP⁺ dynamics for OSKCtr (blue plot) and OSKR (red plot) based on live imaging. (g) Representative of Oct4-GFP⁺ colonies during reprogramming (bar = 2 mm). (h,i) Reprogramming efficiency was increased by Rab32 in TF4 MEFs. (j,k) Rab32 knockdown reduces the number of AP⁺ colonies following the induction of iPSCs in TF4 MEFs. Data in (f,h,j) are represented as M ± SD (n = 3). The results of TF4 + Ctr, TF4 + shCtr and OSKCtr were used as controls respectively. *p < 0.05.