Figure 3
The Overexpression of Rab32 in MEFs Induces a Greater Number of Lipid Droplets and Autophagosomes.
(a) Confocal immunofluorescence microscopy of MEFs double-labeled with polyclonal antibodies against Rab32 (green) (bar = 5 μm, up; bar  = 10 μm, down). (b) Nile red staining of lipid droplets in MEFs (bar = 2 μm). (c) Using Harmony software to calculate the number of lipid droplets, over-expression of Rab32 results in a greater number of lipid droplets number compared to the control (n = 3, the result of MEFCtr was used as control, *p < 0.05). (d) Immunofluorescence staining of LC3+ dots in MEFs (bar = 2 μm). (e) The number of LC3+ dots per cell (n = 3, the result of MEFCtr was used as control, **p < 0.01) was quantified and the overexpression of Rab32 induces more LC3 dots.
