Figure 5

Rab32 Rescues the Reduction in iPSC Generation Produced by Inhibiting Lipid Synthesis during Reprogramming.

(a) Inhibitors of autophagy and lipid synthesis reduce the efficiency of reprogramming with OSK in the OG2 MEFs. The results of DMSO treatment were used as controls and the right side was the representative of AP⁺ colonies (n = 3, *p < 0.05, **p < 0.01,***p < 0.001). (b) Inhibitors of autophagy and lipid synthesis reduce the efficiency of reprogramming in TF4 MEFs. The results of DMSO were used as controls and the right side was the representative of AP⁺ colonies (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001). (c) Rab32 rescues the impact of reducing the programming efficiency caused by TOFA in the OG2 MEFs. The results of TOFA+Ctr were used as controls and the right side was the representative of AP⁺ colonies (n = 3, *p < 0.05). (d) Rab32 cannot rescue the impact of reducing the programming efficiency caused by SP and SP+TOFA in the OG2 MEFs. The results of SP+Ctr and SP+TOFA+Ctr were used as controls (n = 3). (e) Rab32 rescues the reduction in programming efficiency caused by TOFA in TF4 MEFs. The results of TOFA+Ctr were used as controls and the right side is representative of AP⁺ colonies (n = 3, *p < 0.05). (f) Rab32 cannot rescue the impact of reducing the programming efficiency caused by SP and SP+TOFA in the TF4 MEFs. The results of SP+Ctr and SP+TOFA+Ctr were used as controls (n = 3).