Figure 2 | Scientific Reports

Figure 2

From: Absence of DAB2IP promotes cancer stem cell like signatures and indicates poor survival outcome in colorectal cancer

Figure 2

DAB2IP regulates epithelial-mesenchymal transition (EMT) in CRC in vitro.

(A) The stable cell lines were established, SW480 was transfected with either PCI-DAB2IP (D2 and D4) or PCI-neo (control), HT29 and HCT116 with either pGIPZ-shcon(HT29-shcon and HCT116-shcon) or pGIPZ-shDAB2IP(HT29-KD4,KD6 and HCT116-KD2,KD3). E-cadherin, vimentin and DAB2IP were detected, β-actin as loading control. (B) The morphology of the sublines was shown by phase-contrast microscopy (magnification: 100x). (C) EMT related transcriptional factors were analyzed in SW480, HT19 and HCT116 by quantitative RT-PCR. (D) DAB2IP affects the migration in vitro by transwell and quantitative measurement of migratory cell was done.

Back to article page