Figure 3 | Scientific Reports

Figure 3

From: Absence of DAB2IP promotes cancer stem cell like signatures and indicates poor survival outcome in colorectal cancer

Figure 3

DAB2IP regulates the phenotype of cancer stem cell in vitro.

(A) DAB2IP inhibits clone formation in soft-agar. down: quantitative measurement of number and diameter of clones (diameter> = 50 μm); up: the morphology of clones (magnification: 50x). (B) DAB2IP inhibits sphere formation in vitro: left: quantitative measurement of number and diameter of spheres (diameter> = 50 μm); right: morphology of spheres (magnification: 100x); western blot showed that DAB2IP expression in sphere cells. (C) Surface markers and CSC related transcriptional factors are detected by quantitative RT-PCR. (D): SW480 (con and D2),HT29 (shcon and KD6) and HCT116 (shcon and KD2) were treated by oxaliplatin (50 μM) or 5-Fu (200 μg/ml) respectively, after 12 hours, cell death was assayed by PE-Annexin V/7-AAD.

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