Figure 2 | Scientific Reports

Figure 2

From: Catestatin attenuates endoplasmic reticulum induced cell apoptosis by activation type 2 muscarinic acetylcholine receptor in cardiac ischemia/reperfusion

Figure 2

CST pretreatment reduced ER-stress and decreased apoptosis.

(a) Cleaved caspase-3 immunofluorescence staining and nuclear staining; apoptotic cells were stained in pink Scale bar = 100 μM. (b) Apoptosis cell numbers were counted in five different field of vision. (c) Western blot analysis of effect of I/R and CP on apoptosis and caspase family members and PARP and (d) proteins related to the ER stress pathway. Relative protein expression was analyzed by band gray degree. β-actin is a normalization control (e). Six independent experiments were performed for above studies.

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