Figure 1
Effect of quercitrin on hGF stimulated with IL-1β.
(A) Experimental design: the effect of quercitrin (QUER) treatment on hGF was evaluated in four different inflammatory scenarios (stripped pattern); treatment for 1 day with interleukin-1 beta (IL-1β); treatment for 3 days with IL-1β; treatment with IL-1β during the first 3 days and culturing the cells for 14 days (therapeutic approach; T); and culturing the cells for 14 days plus IL-1β treatment during the last 3 days (preventive approach; P). (B) Cytotoxicity measured after 1 and 3 days of treatment. Dotted line represents high control (100% cytotoxicity). (C) Gene expression results: cells were treated with quercitrin (grey bars) or without (white bars), following the experimental design. Data were normalized to reference genes, expressed as percentage of control (vehicle), which was set to 100%. Values represent the mean ± SEM of two independent experiments. One, two and three symbols represent a significant difference between two groups with P ≤ 0.05, P < 0.01 and P < 0.001, respectively: (*) treatment versus control (vehicle); (#) quercitrin versus vehicle in the presence of IL-1β for each time point.
