Figure 6

Overexpression of TMEM16A in an HPV-positive cell line confers no increase in proliferation or survival.
(a) Western Blot showing forced overexpression of TMEM16A in the HPV-positive SCC90 cell line. (b) Representative photo of colony formation assay, showing that forced overexpression of TMEM16A does not confer increased colony formation. (c) Forced overexpression of TMEM16A in the HPV-positive SCC90 cell line does not confer enhanced proliferation. (d,e) In SCC90 cells, forced overexpression of TMEM16A does not result in resistance to CaCCinh, as evidenced by the nearly identical IC50 to the wild type SCC90 cell line.