Figure 4
From: Ligand-driven conformational changes of MurD visualized by paramagnetic NMR
Mapping of the chemical shift perturbations upon ligand binding.
The chemical shift perturbation observed in the backbone amide groups of MurD by the addition of its ligands. (a) Perturbation by the addition of 5 equivalents of AMP-PNP. (b) Perturbation by the addition of 2 equivalents of AMP-PNP in the presence of 20 equivalents of Mg2+. (c) Perturbation by the addition of 5 equivalents of UMA. (d) Perturbation by the addition of 2 equivalents of UMA in the presence of 20 equivalents of Mg2+ and 3 equivalents of AMP-PNP. (e) Perturbation by the addition of 5 equivalents of d-Glu in the presence of 20 equivalents of Mg2+, 5 equivalents of AMP-PNP and 3 equivalents of UMA. (f) Perturbation by the addition of 1 equivalent of compound 1. (g) Perturbation by the addition of 5 equivalent of compound 2.
